38 although uncommon n. Buffered charcoal yeast extract agar base bcye with added supplements is used for the selective culture of legionella species and legionella isolation from clinical and other sampleslegionella species do not form spores and are narrow gram negative rodslegionella causes pneumonia legionnaires disease or a milk febrile disease pontiac feverthey do not oxidise or ferment carbohydrates in conventional media or grow on sheeps blood agargrowth on buffered charcoal yeast extract agar.
Buffered Charcoal Yeast Extract Agar Base Microbiology
Bcye Agar 10 Pkdo Not Freeze Clinical Laboratory Products
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Sterilize by autoclaving at 15 lbs pressure 1210c for 15 minutes.
Buffered charcoal yeast extract. Find millipore b4801 msds related peer reviewed papers technical documents similar products more at sigma aldrich. Mix well and pour with constant. In water and other samples suspected of harboring the bacteria.
Pressure 121 0c for 15 minutes. Buffered charcoal yeast extract bcye agar formulations are recommended for use in the cultivation and primary isolation of legionella spp. Buffered charcoal yeast extract bcye feeley et al in 1978 developed a medium to isolate legionella species which was later modified by substituting yeast extract for casein hydrolysate and beef extract and replacing starch with activated charcoal and named it as charcoal yeast extract cye agar.
Buffered charcoal yeast extract bcye agar is a selective growth medium used to culture or grow certain types of bacteria particularly the gram negative species legionella pneumophila. Pretreatment with an acid wash and inoculation onto selective buffered charcoal yeast extract agar as formulated for the detection of legionella spp can increase the recovery of nocardia from respiratory specimens. Buffered charcoal yeast extract agar base m813 buffered charcoal yeast extract agar base with added supplements is used for selective cultivation of legionella species from clinical and other specimens.
Heat to boiling to dissolve the medium completely. Suspend 20 grams of buffered charcoal yeast extract agar base in 500 mls distilled water. Aseptically add sterile rehydrated contents of 1 vial each of legionella supplement.
Suspend 20 grams in 500 ml distilled water. In water and other samples suspected of harboring the bacteria. Asteroides complex strains can be isolated in automated blood culture systems especially if patients are receiving immunosuppressive therapy.
Buffered charcoal yeast extract agar base. Aseptically add the sterile rehydrated contents of l8785 and l9285 legionella supplements. P251 is not intended to be used for the diagnosis of human disease.
Hardy diagnostics buffered charcoal yeast extract bcye agar formulations are recommended for use in the cultivation and primary isolation of legionella spp. Sterilize by autoclaving at 15 lbs. 1 it has also been used for the laboratory diagnosis of acanthamoeba keratitis 2 francisella and nocardia.
Legionella Lablogatory
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