1 it has also been used for the laboratory diagnosis of acanthamoeba keratitis 2 francisella and nocardia. Legionella causes pneumonia legionnaires disease 1 or a milk febrile disease pontiac fever.
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Legionella Buffered Charcoal Yeast Extract Bcye Agar Base
Thermo scientific remel buffered cye agar buffered charcoal yeast extract bcye is used for the cultivation and isolation of legionella species.
Buffered charcoal yeast extract bcye agar. Sterilise by autoclaving at 1210 c for 15 minutes. Directions to prepare bcye without l cysteine agar suspend 25 g of legionella cye agar base cm0655 in 90 ml of distilled water and bring gently to the boil to dissolve completely. Hardy diagnostics buffered charcoal yeast extract bcye agar formulations are recommended for use in the cultivation and primary isolation of legionella spp.
Bcye agar buffered charcoal yeast extract for legionella 15x100mm plate order by the package of 10 by hardy diagnostics buffered charcoal yeast extract bcye agar formulations are recommended for use in the cultivation and primary isolation of legionella spp. Buffered charcoal yeast extract bcye feeley et al in 1978 developed a medium to isolate legionella species which was later modified by substituting yeast extract for casein hydrolysate and beef extract and replacing starch with activated charcoal and named it as charcoal yeast extract cye agar. In water and other samples suspected of harboring the bacteria.
Suspend 20 grams in 500 ml distilled water. Heat to boiling to dissolve the medium completely. In water and other samples suspected of harboring the bacteria.
Mix well and pour with constant. Aseptically add sterile rehydrated contents of 1 vial each of legionella supplement. The formulation of buffered charcoal yeast extract agar base is as per specification laid in iso 11731 210.
Buffered charcoal yeast extract bcye agar is an enrichment medium used for the isolation of. Ingredients gramslitre yeast extract 100 charcoal activated 20 aces buffer 100 a ketoglutarate monopotassium salt 10 agar 170 final ph at 250c 69 02. Legionella species are non spore forming narrow gram negative rods.
Legionella bcye agar base for selective cultivation of legionella species. 86558 buffered charcoal yeast extract agar base bcye agar base. Sterilize by autoclaving at 15 lbs pressure 1210c for 15 minutes.
A further modification was made by pasculle et al in 1980 by the addition of aces n 2 acetamido 2 aminoethane sulfonic acid buffer in order to maintain the proper ph for optimal growth. Buffered charcoal yeast extract bcye agar is a selective growth medium used to culture or grow certain types of bacteria particularly the gram negative species legionella pneumophila.
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