Add 24 g koh pellets and mix to dissolve. Francisella tularensis can be cultured aerobically using chocolate agar buffered charcoal yeast extract agar thayer martin agar mueller hinton broth etc.
Colonies Bacterium Legionella Pneumophila On Buffered Stock Photo
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Buffered Charcoal Yeast Extract Agar Bcye Agar Ssenw
Buffered charcoal yeast extract bcye feeley et al in 1978 developed a medium to isolate legionella species which was later modified by substituting yeast extract for casein hydrolysate and beef extract and replacing starch with activated charcoal and named it as charcoal yeast extract cye agar.